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Updated: May 21, 2026

A Single-fly Assay for Foraging Behavior in Drosophila
Published on: November 4, 2013
Protocol for simulating foraging behavior strategy and dual-choice predatory sensory cue experiments in
Lixin Gong1, Yang Geng1, Zhiqiang Wang1
1Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun 130117, China; Key Laboratory of Vegetation Ecology of Education Ministry, Institute of Grassland Science, Northeast Normal University, Changchun 130024, China.
Abstract:
The nocturnal and cryptic nature of bats makes it difficult to determine their foraging behavior and predatory sensory cues. Here, we present a protocol for determining the behavioral strategies and sensory cues of bat predation on airborne prey. We describe steps for indoor foraging behavior simulation, dual-choice acoustic playback, and visual presentation experiments. This protocol was used to study the avivorous bat, Ia io, but it can be adapted for studying other aerial-hawking bats and even other taxa. For complete details on the use and execution of this protocol, please refer to Gong et al. (2022).1.

