In vitro evaluation of BMSCs early proliferation on minocycline-loaded electrospun nanofibers membrane
Related Experiment Video
Updated: Jul 20, 2025
Postproduction Processing of Electrospun Fibres for Tissue Engineering
Published on: August 9, 2012
Quan Liu1, Xiao Ma2, Yanchen Pei3
1Institute of Advanced Technology, University of Science and Technology of China, Hefei, China.
Background:
Electrospun nanofibers could simulate the natural extracellular matrix (ECM) of the host bone, while minocycline (MINO) is a broad-spectrum tetracycline antibiotic which has been found to have multiple non-antibiotics biological effects that promotes osteogenesis in vitro and in vivo.
Objective:
The present study aims at constructing a polylactic acid (PLA) electrospun nanofiber membrane loaded with MINO to enhance Bone marrow mesenchymal stem cells (BMSCs) adhesion and proliferation for early clinical treatment.
Methods:
The MINO-PLA membrane were characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR) and in vitro drug release study. The antibacterial ability was also investigated. In addition, in vitro cellular proliferation experiment was performed to verify whether the PLA electrospun nanofibers membrane loaded with MINO enhance BMSCs adhesion and proliferation.
Results:
Analyzing the drug release and cell growth results, it was found that only the effective concentration of MINO-PLA could help the growth of BMSCs in the short term. This is related to the drug release rate of MINO-PLA and the initial concentration of MINO.
Conclusion:
This study shows that by controlling the concentration and release rate of MINO with electrospinning PLA, BMSCs could proliferate on it, and a new bone repair material had been made in this study.
Insights
This study developed a polylactic acid (PLA) nanofiber membrane loaded with minocycline (MINO) to improve bone marrow mesenchymal stem cell (BMSC) growth for bone repair. Controlled MINO release enhances BMSC proliferation for new bone tissue engineering.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Drug Delivery
Background:
- Electrospun nanofibers mimic the natural bone extracellular matrix (ECM).
- Minocycline (MINO) exhibits osteogenic properties beyond its antibiotic effects.
- Polylactic acid (PLA) electrospun nanofibers offer a scaffold for bone regeneration.
Purpose of the Study:
- To construct a PLA electrospun nanofiber membrane loaded with MINO.
- To enhance bone marrow mesenchymal stem cell (BMSC) adhesion and proliferation.
- To develop a novel material for early clinical bone repair.
Main Methods:
- Fabrication and characterization of MINO-loaded PLA nanofiber membranes using SEM and FTIR.
- In vitro drug release kinetics and antibacterial activity assessment.
- Evaluation of BMSC adhesion and proliferation on the MINO-PLA membranes.
Main Results:
- The MINO-PLA membranes demonstrated controlled drug release profiles.
- Effective MINO concentrations promoted short-term BMSC proliferation.
- Drug release rate and initial MINO concentration influenced BMSC growth.
Conclusions:
- Controlling MINO concentration and release rate via electrospun PLA is crucial for BMSC proliferation.
- This study presents a new bone repair material utilizing MINO-loaded PLA nanofibers.
- The developed material shows potential for bone tissue engineering applications.
