Related Experiment Video
Updated: Jul 19, 2025
05:32
3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting
Published on: April 21, 2021
3.1K
3D-Printed Microcubes for Catalase Drug Delivery.
Sungmun Lee1,2, Dong-Wook Lee3, Nitul Rajput3
1Department of Biomedical Engineering, Khalifa University of Science and Technology, Abu Dhabi 127788, UAE.
ACS Omega
|August 7, 2023
Summary
Three-dimensional printed microcubes efficiently deliver catalase, an antioxidant enzyme, protecting cells from oxidative stress. These uniform microparticles show promise for therapeutic protein delivery.
Area of Science:
- Biomedical Engineering
- Materials Science
- Nanotechnology
Background:
- Oxidative stress from reactive oxygen species (ROS) contributes to inflammatory diseases.
- Catalase, an antioxidant enzyme, has therapeutic potential but faces delivery challenges.
- Uniform, small drug delivery vehicles are crucial for efficient therapeutic protein delivery.
Purpose of the Study:
- To investigate 3D-printed microcubes as drug delivery vehicles for catalase.
- To assess the characteristics of 3D-printed microcubes for targeted delivery.
- To evaluate the efficacy of catalase-loaded microcubes.
Main Methods:
- High-resolution 3D printing (Nanoscribe Photonic Professional GT2) to create microcubes.
- Loading catalase onto microcubes via nonspecific adsorption.
- Assessing catalase activity and protective effects on macrophages (RAW 264.7) against hydrogen peroxide (H₂O₂).
Main Results:
- 3D-printed microcubes (800 nm x 600 nm) are suitable for passive macrophage targeting.
- Catalase-loaded microcubes (CAT-MC) retained 83.1% of intact catalase activity.
- CAT-MC protected macrophages from H₂O₂-induced cytotoxicity by 86.4%.
Conclusions:
- 3D-printed microparticles offer tunable size and shape for consistent drug distribution and activity.
- These microparticles are highly promising for delivering therapeutic proteins.
- Potential for broad applications in treating inflammatory diseases.
