Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.2K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Universal diseased-site targeting via glycolysis-driven lactic acid gradient.

Science advances·2026
Same author

ENPP1 blockade with a humanized monoclonal antibody enhances renal repair after acute kidney injury.

Cell stem cell·2026
Same author

Label-free interferometry platform for drug response profiling of bioprinted tumor organoids at single-organoid resolution.

Nature protocols·2026
Same author

Mitochondrial transfer technologies with molecular insights into clinical applications.

Stem cells (Dayton, Ohio)·2026
Same author

Practice and Determinants of Spontaneous Adverse Drug Reaction Reporting Among Healthcare Professionals: A Multicenter Cross-Sectional Study in Vietnam.

Hospital pharmacy·2026
Same author

Characterization of Mitochondrial Double-Stranded RNA Levels in Non-Small Cell Lung Carcinoma.

Cancer research communications·2026

Related Experiment Video

Updated: Jul 19, 2025

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform
08:10

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform

Published on: October 6, 2019

6.6K

Massively Concurrent Sub-Cellular Traction Force Videography enabled by Single-Pixel Optical Tracers (SPOTs).

Xing Haw Marvin Tan, Yijie Wang, Xiongfeng Zhu

    Biorxiv : the Preprint Server for Biology
    |August 7, 2023
    PubMed
    Summary

    We developed a new optical platform to measure cell mechanics. This tool tracks sub-cellular forces in thousands of cells, revealing new insights into cardiac tissue wave behavior.

    More Related Videos

    Directly Measuring Forces Within Reconstituted Active Microtubule Bundles
    07:47

    Directly Measuring Forces Within Reconstituted Active Microtubule Bundles

    Published on: May 10, 2022

    1.7K
    Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
    09:56

    Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

    Published on: August 31, 2021

    4.9K

    Related Experiment Videos

    Last Updated: Jul 19, 2025

    Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform
    08:10

    Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform

    Published on: October 6, 2019

    6.6K
    Directly Measuring Forces Within Reconstituted Active Microtubule Bundles
    07:47

    Directly Measuring Forces Within Reconstituted Active Microtubule Bundles

    Published on: May 10, 2022

    1.7K
    Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
    09:56

    Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

    Published on: August 31, 2021

    4.9K

    Area of Science:

    • Biophysics
    • Cell Biology
    • Bioengineering

    Background:

    • Understanding cell mechanics is crucial for tissue engineering and disease research.
    • Existing methods for measuring cellular traction forces often lack the speed or scale required for complex biological systems.

    Approach:

    • Developed a high-speed, large-field-of-view videography platform using Single-Pixel Optical Tracers (SPOT).
    • SPOT utilizes 2D diffraction gratings in a soft substrate to translate cellular traction stress into optical signals.
    • The platform captures data from over 10,000 cells across 13mm² at 83 frames per second.

    Key Points:

    • Enables measurement of sub-cellular traction forces in diverse cell types, from isolated cells to connected tissue sheets.
    • Successfully applied to study mechanical wave propagation in Neonatal Rat Ventricular Myocytes (NRVMs).
    • Revealed heterogeneous activation times within cardiac tissue, deviating from uniform spiral wave behavior.

    Conclusions:

    • The SPOT platform offers unprecedented capabilities for high-throughput, high-resolution cell mechanics analysis.
    • Provides a novel tool for investigating dynamic cellular processes and tissue-level mechanics.
    • Uncovered previously unobserved heterogeneity in cardiac wave propagation, impacting our understanding of heart function.