Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

X-ray Imaging

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mapping fast tissue dynamics with long camera exposures via intensity modulation.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Weifuchun inhibits gastric cancer metastasis by inhibiting angiogenesis mediated by the miR-139-5p/CXCR4 axis.

Journal of ethnopharmacology·2026

Same author

Multi-exposure speckle imaging through an optical fiber bundle.

Journal of biomedical optics·2025

Same author

Improving vascular retention of indocyanine green for <i>in vivo</i> two-photon microscopy using liposomal encapsulation.

Journal of biomedical optics·2025

Same author

Quality of Life and Body Dissatisfaction in Cisgender Men Reporting Sexual Attraction Toward Men or Women.

Europe's journal of psychology·2024

Same author

Non-degenerate two-photon imaging of deep rodent cortex using indocyanine green in the water absorption window.

Biomedical optics express·2024

Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026

Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026

Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026

Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026

Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026

Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 19, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Wide-field Intensity Fluctuation Imaging.

Qingwei Fang¹, Alankrit Tomar², Andrew K Dunn^1,2

¹Department of Biomedical Engineering, The University of Texas at Austin, Austin, 78712, TX, US.

Biorxiv : the Preprint Server for Biology

|August 7, 2023

Summary

This summary is machine-generated.

Measuring light fluctuations, crucial for optics and biology, is now faster. A new 2-pulse method enables wide-field intensity autocorrelation mapping, overcoming camera speed limits for advanced optical sensing.

Keywords:

autocorrelation function camera intensity fluctuation within-exposure modulation

More Related Videos

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Published on: August 16, 2021

Related Experiment Videos

Last Updated: Jul 19, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Published on: August 16, 2021

Area of Science:

Optics and Photonics
Biomedical Optics
Astrophysics

Background:

Temporal intensity fluctuations provide insights into light sources and light-medium interactions.
Intensity autocorrelation function () is key for applications like stellar interferometry, fluorescence correlation spectroscopy, and dynamic light scattering.
Single-point measurement is feasible with high-frequency sampling, but wide-field measurement is limited by camera frame rates.

Conclusions:

The proposed 2-pulse modulation method effectively breaks the camera frame rate barrier for wide-field measurements.
This advancement facilitates broader applications of intensity autocorrelation analysis in various scientific fields.