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Updated: Jul 19, 2025

A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication

A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication

Published on: September 20, 2016

RobustCCC: a robustness evaluation tool for cell-cell communication methods.

Chenxing Zhang¹, Lin Gao¹, Yuxuan Hu¹

¹School of Computer Science and Technology, Xidian University, Xi'an, China.

Frontiers in Genetics

|August 7, 2023

Summary

This summary is machine-generated.

RobustCCC evaluates cell-cell communication (CCC) inference tools, finding method performance varies significantly with input data. This tool helps select robust CCC methods for analyzing tissue microenvironments.

Keywords:

RobustCCC cell-cell communication evaluation robustness scRNA-seq

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Last Updated: Jul 19, 2025

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Published on: December 1, 2018

Area of Science:

Computational Biology
Bioinformatics
Genomics

Background:

Cell-cell communication (CCC) inference is crucial in single-cell data analysis.
Numerous computational tools exist for CCC inference, but their robustness is not well understood.

Purpose of the Study:

To develop RobustCCC, a user-friendly tool for evaluating the robustness of CCC inference methods.
To assess CCC method performance against data replication, noise, and prior knowledge variations.

Main Methods:

Integrated 14 state-of-the-art CCC methods.
Utilized 6 simulated single-cell transcriptomics datasets for evaluation.
Generated tabular reports detailing robustness performance.

Main Results:

CCC methods showed significantly different robustness across various simulation datasets.
Input data characteristics strongly influence inferred CCC patterns.
Identified variability in method performance highlights the need for robustness assessment.

Conclusions:

RobustCCC provides a scalable platform for evaluating and comparing CCC inference tools.
The tool aids in selecting stable and consistent CCC methods for tissue microenvironment analysis.
Facilitates informed choices for robust cell-cell communication identification.