Method for the Rapid Detection of SARS-CoV-2-Neutralizing Antibodies Using a Nanogel-Based Surface Plasmon Resonance...

Area of Science:

• Immunology
• Biotechnology
• Nanotechnology

Background:

• COVID-19 vaccine efficacy correlates with neutralizing antibody (NAb) levels targeting the SARS-CoV-2 spike protein receptor-binding domain (RBD).
• Accurate NAb measurement is crucial for vaccine development and achieving population immunity.
• The gold standard plaque reduction neutralization test is limited by its requirement for high-level biosafety containment.

Purpose of the Study:

• To develop a rapid, quantitative, and safe platform for detecting SARS-CoV-2 NAbs in clinical samples.
• To overcome the limitations of traditional NAb detection methods for wider application.

Main Methods:

• Development of a bioresponsive nanogel-based surface plasmon resonance (nSPR) platform.
• Conjugation of RBD protein to nanogels for enhanced NAb detection.
• Utilizing multivalent protein binding (MPB) for signal amplification and specificity.

Main Results:

• The nSPR platform demonstrated high selectivity for NAbs, resisting interference from other serum proteins.
• Significantly enhanced SPR signals were observed due to MPB between nanogel-RBD and NAbs.
• The assay allows for rapid and precise detection and quantification of SARS-CoV-2 NAbs.

Conclusions:

• The nanogel-based nSPR assay provides a sensitive, specific, and user-friendly method for NAb detection.
• This platform facilitates easier and safer COVID-19 vaccination strategies and monitoring.
• It offers a viable alternative to conventional methods, enabling broader clinical and research applications.