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Updated: Jul 19, 2025
07:31
A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate
Published on: May 3, 2021
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CircECE1 promotes osteosarcoma progression through regulating RAB3D by sponging miR-588
Zhizhong Liang1, Yuxia Shi1, Zhe Guan2
1Department of Bone and Soft Tissue Oncology, Shanxi Province Cancer Hospital, Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences, Cancer Hospital Affiliated to Shanxi Medical University, No.3, Zhigong New Street, Xinghualing District, Taiyuan, 030013, China.
Journal of Orthopaedic Surgery and Research
|August 9, 2023
Summary
Circular RNA circECE1 promotes osteosarcoma progression by sponging miR-588, which targets RAB3D. Silencing circECE1 inhibits tumor growth, revealing a novel circECE1/miR-588/RAB3D axis in osteosarcoma.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Circular RNAs (circRNAs) play a role in cancer development.
- The specific role of circRNA endothelin converting enzyme 1 (circECE1) in osteosarcoma (OS) remains unclear.
Purpose of the Study:
- To investigate the mechanism of circECE1 in osteosarcoma (OS) progression.
- To elucidate the circECE1/miR-588/RAB3D axis in OS.
Main Methods:
- Quantitative real-time PCR and western blot to measure expression levels.
- Cell proliferation, apoptosis, and metastasis assays (CCK-8, EdU, flow cytometry, transwell).
- Dual-luciferase reporter, RNA immunoprecipitation, and xenograft models to confirm interactions and in vivo function.
Main Results:
- circECE1 expression is elevated in OS tissues and linked to increased cell viability, proliferation, migration, and invasion.
- circECE1 acts as a sponge for miR-588, and miR-588 targets RAB3D, regulating OS progression.
- Silencing circECE1 inhibited OS cell progression and tumor growth in vivo.
Conclusions:
- circECE1 promotes osteosarcoma progression through the miR-588/RAB3D pathway.
- The circECE1/miR-588/RAB3D axis represents a potential therapeutic target for osteosarcoma.
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