Cell-free synthesis of high-molecular-weight small intestinal polypeptides

An intrinsic membrane glycoprotein, Mr 131 000, is a major developmentally specific component of the neonatal guinea-pig small intestinal microvillar membrane. Such high-molecular-weight proteins are often difficult to translate in vitro. In this study we report a successful strategy for the identification of the primary translation product of this glycoprotein, a high-molecular-weight precursor polypeptide of approximate Mr 225 000.