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Related Concept Videos

Histone Modification02:32

Histone Modification

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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
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We developed a faster, droplet-based Paired-Tag method to map histone modifications and gene expression in single cells. This improved technique aids in identifying cis-regulatory elements and their links to gene expression in complex tissues.

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Area of Science:

  • Genomics
  • Epigenetics
  • Single-cell biology

Background:

  • Paired-Tag enables simultaneous mapping of histone modifications and gene expression at single-cell resolution.
  • The original Paired-Tag protocol's lengthy procedure limited its widespread adoption.
  • A need exists for more accessible and efficient methods for multi-omic single-cell analysis.

Purpose of the Study:

  • To develop a faster and more accessible droplet-based Paired-Tag protocol.
  • To overcome the procedural bottlenecks of the original Paired-Tag method.
  • To enable large-scale, simultaneous analysis of chromatin state and gene expression in single cells.

Main Methods:

  • Development of a droplet-based microfluidic system for Paired-Tag.
  • Adaptation of combinatorial indexing for high-throughput single-cell multi-omics.
  • Application of the protocol to cultured mammalian cells and primary brain tissues.

Main Results:

  • The droplet-based Paired-Tag protocol significantly reduces experimental time and complexity.
  • Demonstrated superior performance in identifying candidate cis-regulatory elements.
  • Successfully associated dynamic chromatin states with target gene expression in individual cells within complex tissues.

Conclusions:

  • The droplet-based Paired-Tag protocol is a faster and more accessible method for single-cell multi-omic analysis.
  • This method enhances the ability to study epigenomic regulation of gene expression at single-cell resolution.
  • The improved protocol facilitates broader application in complex biological systems.