Related Experiment Video
Updated: Jul 19, 2025
09:58
Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
Published on: June 27, 2020
2.8K
Integrative Transcriptomic Profiling of the Wilms Tumor.
Simona Lucija Avčin1,2, Klementina Črepinšek2,3, Barbara Jenko Bizjan2,3
1Department of Haematology and Oncology, University Children's Hospital, University Medical Centre Ljubljana (UMC), 1000 Ljubljana, Slovenia.
Cancers
|August 12, 2023
Summary
This study identified novel microRNA (miRNA) and messenger RNA (mRNA) biomarkers for Wilms tumor, a common pediatric kidney cancer. These transcriptomic biomarkers show potential for diagnosing and treating Wilms tumor, irrespective of its type or stage.
Area of Science:
- Oncology
- Genomics
- Molecular Biology
Background:
- Wilms tumor is the most common pediatric kidney cancer.
- Identifying reliable biomarkers independent of histological type and stage is crucial for effective diagnosis and treatment.
- Understanding the transcriptomic landscape, including miRNA and mRNA interplay, can elucidate Wilms tumor pathogenesis.
Purpose of the Study:
- To identify transcriptomic biomarkers for Wilms tumor.
- To analyze miRNA and mRNA profiles and their interplay in Wilms tumor.
- To discover biomarkers applicable regardless of histological subtype and disease stage.
Main Methods:
- Next-generation sequencing was used to analyze miRNA profiles of 74 kidney samples (fresh frozen and FFPE).
- mRNA expression profiling and pathway analysis were conducted.
- Bioinformatics approaches were employed for comparative analysis and identification of differentially expressed genes and miRNAs.
Main Results:
- 41 differentially expressed miRNAs were identified between Wilms tumor and healthy kidney tissue.
- 27 miRNAs showed decreased expression, while 14 exhibited increased expression in Wilms tumor.
- A limited list of genes potentially regulated by differentially expressed miRNAs in Wilms tumor was suggested.
Conclusions:
- The study identified a comprehensive miRNA and mRNA profile for Wilms tumor.
- The findings provide insights into the pathogenesis of Wilms tumor.
- Identified miRNAs hold potential as diagnostic and therapeutic biomarkers for Wilms tumor, irrespective of subtype and stage.
