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Updated: Jul 19, 2025
10:24
Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
Published on: March 11, 2018
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Oxido-Reduction Potential as a Method to Determine Oxidative Stress in Semen Samples.
András Balló1,2,3, Péter Czétány2,3, Kinga Székvári Busznyákné1
1Pannon Reproduction Institute, 8300 Tapolca, Hungary.
International Journal of Molecular Sciences
|August 12, 2023
Summary
Oxidative stress (OS) impacts male infertility. Oxido-reduction potential (ORP) measurement can detect reactive oxygen species (ROS) but requires normalization for accurate results, especially with varying sperm counts.
Area of Science:
- Reproductive medicine andrology
- Oxidative stress and male reproductive health
Background:
- Infertility affects 15% of couples globally, with oxidative stress (OS) and reactive oxygen species (ROS) implicated in poor semen quality.
- Current diagnostic and treatment protocols for andrological OS are lacking.
- Oxido-reduction potential (ORP) measurement offers a rapid method for assessing OS.
Purpose of the Study:
- To validate correlations between ORP values and sperm parameters.
- To investigate the influence of sperm concentration on ORP measurements.
- To determine if induced intracellular ROS formations are reflected in ORP values.
Main Methods:
- Intracellular ROS were induced using menadione, hydrogen peroxide, and tert-butyl hydroperoxide.
- Sperm motility and viability were assessed using the SCA Scope system.
- ORP changes were monitored using the Mioxsys system.
Main Results:
- Significant correlations were found between ORP, sperm concentration, motility, progressive motility, and viability, but only when ORP was normalized by sperm count.
- Artificial ROS induction decreased sperm viability and motility, altering ORP levels.
- Seminal plasma demonstrated a protective effect, with its removal increasing sperm sensitivity to OS inducers and ORP levels.
Conclusions:
- ORP measurement is a viable tool for detecting intracellular ROS accumulation in andrology.
- Normalization of ORP values by sperm count is crucial for accurate interpretation, as extreme concentrations can be misleading.
- Further research is needed to clarify the limitations of ORP measurement in clinical practice.
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