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Integrated Transcriptome Analysis Identified Key Expansin Genes Associated with Wheat Cell Wall, Grain Weight and
Juan P Mira1, Anita Arenas-M1,2, Daniel F Calderini3
1Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 5110566, Chile.
Plants (Basel, Switzerland)
|August 12, 2023
Summary
This study reveals how wheat expansin genes are expressed during grain development. Certain expansin genes negatively impact wheat grain size and weight, offering targets for crop improvement.
Area of Science:
- Plant Biology
- Genetics
- Agricultural Science
Background:
- Expansins are key cell wall-loosening proteins crucial for plant growth.
- Understanding gene expression dynamics in crop development is vital for yield enhancement.
Purpose of the Study:
- To investigate the temporal expression patterns of expansin genes during wheat (Triticum aestivum L.) grain development.
- To identify correlations between expansin gene expression and grain morphology traits.
Main Methods:
- Comprehensive meta-analysis of public RNA-seq data.
- Identification and temporal analysis of differentially expressed genes (DEGs).
- Hierarchical clustering, co-expression network analysis, and quantitative PCR (qPCR) validation.
Main Results:
- Identified 60,852 DEGs, with 28,558 showing significant temporal regulation.
- 30% of known expansin genes were differentially expressed during grain development.
- Expansin genes co-expressed with cell wall modification genes; select genes negatively correlated with grain size and weight.
Conclusions:
- Expansin genes play a significant role in wheat grain development.
- Temporal expression of expansins influences grain morphology.
- Targeting specific expansin genes offers potential for improving wheat yield and quality.
Keywords:
Triticum aestivumcell wall modificationexpansin genesgene co-expression networkwheat grain developmentMore Related Videos
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