Related Experiment Video
Updated: Aug 3, 2026
10:08
Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
Published on: October 21, 2009
21.6K
Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell
Vera Chernonosova1, Marianna Khlebnikova1, Victoriya Popova1
1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, Russia.
Polymers
|August 12, 2023
Summary
This study developed silica nanoparticle-loaded electrospun scaffolds for localized gene delivery in tissue engineering. These scaffolds effectively transfect cells without significant cytotoxicity, showing promise for regenerative medicine applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Gene Delivery Systems
Background:
- Localized gene delivery using biomaterials is crucial for developing advanced tissue engineering scaffolds.
- Cell transfection within scaffolds can induce protein production or cell reprogramming for regenerative purposes.
Purpose of the Study:
- To create and evaluate silica nanoparticles-loaded with plasmid DNA (pDNA) integrated into electrospun scaffolds.
- To assess pDNA release, cell-scaffold interactions, cell viability, and transfection efficacy.
Main Methods:
- Synthesis and characterization of pDNA-coated silica nanoparticles (using dynamic light scattering and transmission electron microscopy).
- Fabrication and characterization of electrospun scaffolds loaded with pDNA-nanoparticles (using scanning electron microscopy, X-ray photoelectron spectroscopy, and stress-loading tests).
- Evaluation of cell viability and pDNA transfection efficiency with HEK293T cells.
Main Results:
- Nanoparticle sizes ranged from 56 to 78 nm, suitable for cell transfection.
- Scaffold properties and pDNA release varied with composition.
- Scaffolds showed no significant cytotoxic effects and demonstrated good potential for cell transfection, particularly the (pDNA-NPs) + PEI9 loaded scaffold.
Conclusions:
- Electrospun scaffolds loaded with pDNA-nanoparticles are viable biomaterials for localized gene delivery.
- These scaffolds support cell viability and facilitate effective cell transfection, making them suitable for tissue engineering applications.
