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USP19 Negatively Regulates p53 and Promotes Cervical Cancer Progression
Apoorvi Tyagi1, Janardhan Keshav Karapurkar1, Jencia Carminha Colaco1
1Graduate School of Biomedical Science and Engineering, Hanyang University, 04763, Seoul, South Korea.
Molecular Biotechnology
|August 12, 2023
Summary
USP19 negatively regulates the tumor suppressor p53 in cervical cancer. Its depletion increases p53 levels, inhibiting cancer cell growth, migration, and invasion.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- p53 is a crucial tumor suppressor gene regulating cell cycle and apoptosis.
- Protein levels of p53 are tightly controlled by E3 ligases and deubiquitinating enzymes (DUBs).
- Several DUBs promote p53 degradation, contributing to tumor progression.
Purpose of the Study:
- To investigate the role of USP19 as a regulator of p53 protein levels.
- To determine the impact of USP19 on cervical cancer progression.
Main Methods:
- Pull-down assays to confirm USP19-p53 interaction.
- Overexpression and CRISPR/Cas9-mediated knockout of USP19 in cervical cancer cells.
- Assessment of p53 ubiquitination, protein half-life, colony formation, migration, and invasion.
Main Results:
- USP19 directly interacts with p53.
- USP19 overexpression enhances p53 ubiquitination and reduces its half-life.
- USP19 knockout increases p53 levels, inhibiting cervical cancer cell proliferation, migration, and invasion.
Conclusions:
- USP19 acts as a negative regulator of p53 protein levels.
- USP19 promotes cervical cancer progression by degrading p53.
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