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Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
Published on: November 30, 2016
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Potential-resolved electrochemiluminescence biosensor for simultaneous determination of multiplex miRNA
Yina Sun1, Shuo Ge2, Ruifang Liu1
1Institute for Advanced Interdisciplinary Research(iAIR), University of Jinan, Jinan, 250022, PR China.
Talanta
|August 12, 2023
Summary
This study introduces a novel electrochemiluminescence (ECL) strategy for simultaneous dual-biomarker detection. The method overcomes cross-interference challenges, enabling sensitive and accurate analysis of microRNAs.
Area of Science:
- Analytical Chemistry
- Biomarker Detection
- Electrochemistry
Background:
- Potential-resolved electrochemiluminescence (ECL) is crucial for multi-target detection but limited by luminophore availability.
- Developing new ECL luminophores is essential for advancing simultaneous detection strategies.
Purpose of the Study:
- To develop a novel ECL strategy for simultaneous detection of dual biomarkers.
- To overcome cross-interference and coreactant competition in multi-target ECL assays.
- To enhance the sensitivity and accuracy of biomarker detection using resolved emission potentials.
Main Methods:
- Utilized polyethyleneimine functionalized perylene derivatives (PTC-PEI) and luminol functionalized gold nanoparticles (Lu-Au NPs) as ECL luminophores.
- Constructed a ternary ECL system with MoS2 nanoflowers and K2S2O8 for cathode ECL enhancement.
- Employed ZnO nanoflowers as an anode coreaction accelerator to enhance anode ECL emission.
Main Results:
- Achieved significantly resolved emission potentials for PTC-PEI and Lu-Au NPs.
- Demonstrated accurate simultaneous detection of miRNA-205 and miRNA-21 over a wide concentration range (10 fM to 100 nM).
- Obtained low detection limits of 2.57 fM for miRNA-205 and 1.15 fM for miRNA-21.
Conclusions:
- The proposed strategy enables single-step synchronous detection of dual biomarkers, addressing key technical challenges.
- This work offers a new approach for ECL detection of multiple biomarkers.
- The developed method holds potential value for clinical diagnosis and analytical science.
