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Updated: Jul 19, 2025
Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
Hinge-like paper-based dual-channel enzyme-free ratiometric fluorescent microfluidic platform for simultaneous visual
Yangyu Yang1, Chaoying Tong2, Rongrong Zhou3
1College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, Hunan, China; Institute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, Changsha 410013, Hunan, China.
Abstract:
On-site multi-pesticide residues detection is particularly urgent and challenging. Here, we fabricated an enzyme-free ratiometric fluorescent detection system in combination with a hinge-like dual-channel 3D microfluidic paper analytical device (3D μPAD) for simultaneous visual detection of carbaryl and glyphosate. Blue-emission 1-naphthol (Em. 470 nm) was hydrolyzed from carbaryl, while yellow-emission 2,3-diaminophenazine (Em. 570 nm) was produced with the aid of Cu2+ for glyphosate sensing. Inner-filter effect between 1-naphthol or 2,3-diaminophenazine and green-emission carbon dots (Em. 510 nm) realized two ratiometric fluorescent detection systems. Remarkable color variation of green-blue for carbaryl (50.00-1100 μΜ) and yellow-green for glyphosate (5.00-600 μΜ) were observed on a dual-channel 3D μPAD without crosstalk. Their detection limits were 1.11 and 0.63 μΜ, respectively. The strategy realized simultaneous visual detection of carbaryl and glyphosate in food/herbal with excellent accuracy (spiked recoveries, 91.00-107.2%), high precision (RSD ≤ 8.43%), and superior selectivity.
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