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Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
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Generation of Recombinant Nucleosomes Containing Site-Specific DNA Damage

Benjamin J Ryan1, Tyler M Weaver1, Jonah J Spencer1

  • 1Department of Biochemistry and Molecular Biology, Department of Cancer Biology, University of Kansas Medical Center, Kansas City, KS, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 13, 2023
PubMed
Summary

Researchers developed a new protocol to create nucleosomes with specific DNA damage. This tool aids in studying DNA repair mechanisms within the complex chromatin environment.

Keywords:
Base excision repairChromatinDNA damageNucleosomes

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