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Stratifying Ferroptosis Sensitivity in Cells and Tissues with PALP
Fengxiang Wang1,2,3, Nathchar Naowarojna1,2,3,4, Yilong Zou5,6,7,8
1Westlake Four-Dimensional Dynamic Metabolomics (Meta4D) Laboratory, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China.
Methods in Molecular Biology (Clifton, N.J.)
|August 14, 2023
Summary
Ferroptosis, a cell death pathway, shows promise for cancer treatment. A new method called photochemical activation of membrane lipid peroxidation (PALP) accurately measures ferroptosis sensitivity in cells and tissues.
Area of Science:
- Biomedical Sciences
- Oncology
- Cell Biology
Background:
- Ferroptosis is an emerging cancer suppression strategy.
- Accurate assessment of ferroptosis sensitivity is crucial for developing targeted therapies.
Purpose of the Study:
- To detail the experimental methodology for Photochemical Activation of Membrane Lipid Peroxidation (PALP) analysis.
- To enable rapid and accurate assessment of ferroptosis sensitivity in biological samples.
Main Methods:
- Preparation of tissue sections and fluorescent lipid peroxidation reporters.
- Sample staining and laser-induced lipid peroxidation.
- Data processing for PALP analysis.
Main Results:
- Photochemical activation of membrane lipid peroxidation (PALP) can efficiently report ferroptosis sensitivity.
- The method is applicable to live cells and tissues in situ.
Conclusions:
- The described PALP analysis provides a detailed protocol for assessing ferroptosis sensitivity.
- This method has potential applications in basic research and clinical investigations for cancer therapy.
