TAMOF-1 as a Versatile and Predictable Chiral Stationary Phase for the Resolution of Racemic Mixtures.

Area of Science:

• Materials Science
• Analytical Chemistry
• Chemical Engineering

Background:

• Metal-organic frameworks (MOFs) offer tunable properties for diverse applications.
• Chiral separation is crucial in the pharmaceutical industry for isolating drug enantiomers.

Purpose of the Study:

• To demonstrate the efficacy of TAMOF-1 as a chiral stationary phase (CSP) in HPLC.
• To validate the use of computational modeling in predicting chiral separations.

Main Methods:

• Utilized a combination of in silico computational modeling and experimental high-performance liquid chromatography (HPLC).
• Developed and tested HPLC columns packed with TAMOF-1 as the chiral stationary phase.
• Separated nine diverse racemic mixtures to assess column performance.

Main Results:

• TAMOF-1 HPLC columns showed efficient, versatile, robust, and reusable chiral separation capabilities.
• Successful resolution of nine racemic mixtures with varying molecular characteristics was achieved.
• In silico screening accurately predicted plausible separations for multiple chiral compound families.

Conclusions:

• TAMOF-1 is a highly effective chiral stationary phase for HPLC.
• Integrated computational and experimental approaches significantly advance chiral chromatography.
• TAMOF-1 columns offer a robust solution for pharmaceutical chiral separations.