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Updated: Jul 19, 2025

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

A new phosphate purification method for igneous weathering profiles.

Chengguo Guan¹, Yongbo Peng^2,3, Ziran Wei⁴

¹State Key Laboratory of Palaeobiology and Stratigraphy, Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences, Nanjing, China.

Rapid Communications in Mass Spectrometry : RCM

|August 14, 2023

Summary

This summary is machine-generated.

A new R-Ca-R phosphate purification method effectively extracts oxygen isotopes from igneous rocks. This method ensures high yields and reproducible paleotemperature data, overcoming limitations of previous techniques.

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Assaying for Inorganic Polyphosphate in Bacteria

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Published on: January 21, 2019

Area of Science:

Geochemistry
Isotope Geochemistry
Biogeochemistry

Background:

Oxygen isotope composition of phosphate (δ18OPO4) is crucial for paleotemperatures and phosphorus cycling.
Existing methods fail for igneous rocks and weathering profiles, necessitating a new approach.

Purpose of the Study:

To develop and validate a reliable phosphate purification method for igneous materials.
To enable accurate δ18OPO4 measurements in igneous rocks and their weathering profiles.

Main Methods:

Phosphate purification using two cation exchange steps and calcium phosphate precipitation (R-Ca-R method).
Improved silver phosphate precipitation with controlled slow evaporation.
Method validation on various rock types and solutions.

Main Results:

Over 99.9% phosphate conversion to calcium phosphate, easily decalcified.
High phosphate yields (97.1%-99.5%) with preserved δ18OPO4 values.
Reproducible δ18OPO4 measurements (±0.3‰) obtained from igneous rocks and profiles.

Conclusions:

A novel phosphate purification method is effective for igneous rocks and weathering profiles.
A reliable indicator for silver phosphate precipitation termination was identified.
The method provides high yields and reproducible δ18OPO4 values for paleoclimate and biogeochemical studies.