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Related Experiment Video

Updated: Jul 19, 2025

Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy
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Harnessing dual-energy CT for glycogen quantification: a phantom analysis.

Meiqin Li1, Zhoulei Li1, Luyong Wei1

  • 1Department of Radiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Quantitative Imaging in Medicine and Surgery
|August 15, 2023
PubMed
Summary

Dual-energy computed tomography (DECT) can accurately quantify glycogen levels in vitro. This non-invasive imaging technique shows excellent linearity, stability, and reproducibility, paving the way for improved diagnostics in glycogen storage disorders.

Keywords:
Glycogendual-energy computed tomography (DECT)quantification

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Area of Science:

  • Medical Imaging
  • Biomedical Engineering
  • Biochemistry

Background:

  • Non-invasive glycogen quantification is vital for understanding glycogen storage disorders.
  • Dual-energy computed tomography (DECT) offers a potential method for in vivo glycogen measurement.

Purpose of the Study:

  • To assess the feasibility of quantifying glycogen content in vitro using DECT.
  • To establish a calibration curve for glycogen concentration measurement with DECT.

Main Methods:

  • A phantom with varying glycogen and iodine concentrations was scanned using fast kilovolt-peak switching DECT.
  • Virtual glycogen concentration (VGC) was measured and correlated with nominal glycogen concentration (NGC).
  • Quantitative performance metrics including linearity, stability, sensitivity, and reproducibility were evaluated.

Main Results:

  • DECT demonstrated an excellent linear relationship between VGC and NGC (R²: 0.989-0.997).
  • High stability, sensitivity (LOD: 6.421-15.315 mg/mL), and reproducibility (ICC: 0.977-0.991) were observed.
  • Low variability (CV: 0.1-0.2%) and consistent conversion factors were achieved across different doses and iodine concentrations.

Conclusions:

  • Phantom studies confirm DECT's efficacy for accurate glycogen detection and quantification.
  • DECT exhibits robust quantitative performance, stability, and reproducibility.
  • DECT holds promise as a convenient tool for glycogen quantification, aiding clinical decision-making.