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Open tubular liquid chromatography: Recent advances and future trends
Deyber Arley Vargas Medina1, Alessandra Timoteo Cardoso1, João Victor Basolli Borsatto1
1Departamento de Química e Física Molecular, São Carlos Institute of Chemistry, University of São Paulo, São Carlos, São Paulo, Brazil.
Journal of Separation Science
|August 15, 2023
Summary
Open tubular liquid chromatography (OTLC) offers enhanced performance over packed columns. This review details recent advancements in OTLC instrumentation and column technology, highlighting its growing applicability in analytical science.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Nano-liquid chromatography (nanoLC) is a key analytical technique, typically using particle-packed columns.
- Open tubular liquid chromatography (OTLC) offers superior performance but faces instrumental challenges, especially with narrow columns (<10 μm i.d.).
Purpose of the Study:
- To provide an updated review of recent developments in Open Tubular Liquid Chromatography (OTLC).
- To focus on instrumental challenges, technological advancements, and applications of OTLC.
Main Methods:
- Review of recent literature on OTLC instrumentation and column technology.
- Analysis of advancements in narrow and larger bore OTLC columns (10-75 μm i.d.).
Main Results:
- Significant progress has been made in developing OTLC systems, including those with very narrow columns (<2 μm i.d.).
- Larger bore OTLC columns (10-75 μm i.d.) show practical applicability.
- Interest in OTLC has resurged due to its perceived advantages.
Conclusions:
- OTLC technology is advancing, overcoming previous instrumental limitations.
- OTLC shows great potential for both targeted and untargeted analytical studies.
- Further developments in OTLC column technology and instrumentation are expected.
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