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Related Experiment Video

Updated: Jul 19, 2025

Generation of Recombinant Influenza Virus from Plasmid DNA

Generation of Recombinant Influenza Virus from Plasmid DNA

Published on: August 3, 2010

Replication-competent influenza virus with a protein-responsive multiplication ability.

Shinzi Ogasawara¹

¹Department of Biology, Faculty of Science, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.

New Biotechnology

|August 16, 2023

Summary

Researchers developed a novel influenza A virus (IAV) system for targeted virotherapy. This replication-competent IAV selectively targets tumor cells by regulating its RNA-dependent RNA polymerase activity via nanobody interactions.

Keywords:

Cell-targeting Influenza virus Nanobody Viral vector Virotherapy

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Last Updated: Jul 19, 2025

Generation of Recombinant Influenza Virus from Plasmid DNA

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Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

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A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection

A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection

Published on: August 14, 2019

Area of Science:

Virology
Biotechnology
Gene Therapy

Background:

Influenza A viruses (IAV) show promise for virotherapy and biotechnology, especially replication-competent strains.
Controlling IAV replication in a cell-type-specific manner is crucial to mitigate risks of uncontrolled infection.

Purpose of the Study:

To develop a simple and effective replication-competent IAV-based cell-targeting system.
To enable precise control over IAV multiplication for targeted therapeutic applications.

Main Methods:

Engineered a system to regulate the activity of the IAV ribonucleoprotein complex (RNP).
Utilized nanobodies fused to the RNA-dependent RNA polymerase (RdRp) subunit to interact with endogenous cellular proteins.
Validated the system using a nanobody against p53 (Nb139) to control RNP activity in p53-expressing versus p53-defective cells.

Main Results:

Demonstrated that RNP activity is inhibited in cells expressing the target protein (p53) and active in cells lacking it.
Successfully generated a replication-competent IAV that selectively replicates in p53-defective tumor cells.
Developed an IAV vector capable of delivering foreign genes in a cell type-specific manner.

Conclusions:

The developed IAV system offers a flexible platform for targeted virotherapy and biotechnology.
The nanobody-based regulation mechanism allows for easy adaptation to target different cell types.
This approach provides a valuable tool for precise and safe viral vector applications.