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Related Experiment Videos

Electrical stability of artificial membranes.

Vladimirov YuA, T V Puchkova, A V Putvinsky

    General Physiology and Biophysics
    |June 1, 1986
    PubMed
    Summary

    Lipid membrane breakdown potential decreases with UV damage, certain enzymes, and pressure. Cholesterol, however, enhances membrane stability against electrical breakdown.

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    Membrane & cell biology·1999

    Area of Science:

    • Biophysics
    • Membrane Biology
    • Biochemistry

    Background:

    • Planar lipid membranes and liposomes are crucial models for cellular membranes.
    • Understanding membrane electrical breakdown potential is vital for cell physiology and pathology.

    Purpose of the Study:

    • To investigate factors influencing the electrical breakdown potential of lipid membranes.
    • To explore the role of lipid peroxidation, enzymatic action, and physical stress on membrane integrity.

    Main Methods:

    • Studied electrical breakdown potential in planar lipid membranes and liposomes.
    • Utilized UV-irradiation, phospholipase A2, protamine sulfate, hydrostatic pressure, and cholesterol treatments.
    • Monitored liposome swelling and turbidity changes to assess membrane rupture.

    Main Results:

    • UV-induced lipid peroxidation, phospholipase A2, protamine sulfate, and hydrostatic pressure decreased membrane breakdown potential.
    • Cholesterol was found to increase the electrical breakdown potential of liposomal membranes.
    • Detergents and lipid peroxidation products further reduced membrane stability.

    Conclusions:

    • Various factors, including oxidative stress and enzymatic activity, significantly impact lipid membrane electrical breakdown.
    • Cholesterol plays a protective role in maintaining membrane integrity against electrical breakdown.
    • The electrical breakdown phenomenon may have implications in cellular pathology.

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