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microRNA-92b-3p augments colon cancer development through inhibiting KLF3

Xuezhong Liu1, Lei Zhang2

  • 1Department of Gastrointestinal Surgery, Liaocheng People's Hospital, Liaocheng, Shandong, China.

Journal of Biochemical and Molecular Toxicology
|August 19, 2023
PubMed

Insights

MicroRNA-92b-3p (miR-92b-3p) promotes colon cancer (CC) development by inhibiting Kruppel-like factor 3 (KLF3). Targeting miR-92b-3p offers a potential therapeutic strategy for CC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Colon cancer (CC) is a significant health concern, with aberrant microRNA expression implicated in its tumorigenesis.
  • MicroRNA-92b-3p (miR-92b-3p) has been observed to be frequently deregulated in various cancers, including colon cancer.

Purpose of the Study:

  • To investigate the specific role of miR-92b-3p in the development and progression of colon cancer.
  • To elucidate the molecular mechanism underlying miR-92b-3p's function in colon cancer, focusing on its interaction with Kruppel-like factor 3 (KLF3).

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) was used to assess miR-92b-3p and KLF3 expression levels in colon cancer tissues and cell lines.
  • In vitro experiments involved transfecting colon cancer cells with miR-92b-3p inhibitors or KLF3 overexpression vectors to evaluate effects on cell proliferation, invasion, migration, and apoptosis.
  • Dual-luciferase reporter assays and Western blotting were employed to validate the targeting relationship between miR-92b-3p and KLF3.
  • Rescue experiments were conducted using co-transfection of miR-92b-3p inhibitors and KLF3 siRNA to confirm the functional interaction.

Main Results:

  • Colon cancer tissues and cells exhibited significantly higher expression of miR-92b-3p and lower expression of KLF3 compared to normal controls.
  • Inhibition of miR-92b-3p or overexpression of KLF3 significantly suppressed colon cancer cell proliferation, invasion, and migration while promoting apoptosis.
  • KLF3 was confirmed as a direct target gene of miR-92b-3p.
  • The observed anti-tumor effects of miR-92b-3p inhibition were reversed upon KLF3 depletion, highlighting KLF3's critical role in mediating miR-92b-3p's function.

Conclusions:

  • miR-92b-3p promotes colon cancer progression by downregulating the expression of its target gene, KLF3.
  • The miR-92b-3p/KLF3 axis represents a potential therapeutic target for the treatment of colon cancer.
  • Understanding this molecular pathway provides a novel avenue for developing targeted therapies against colon cancer.
Keywords:
Kruppel-like factor 3apoptosiscolon cancerinvasionmicroRNA-92b-3pmigrationproliferation

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