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Updated: Jul 18, 2025

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

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Published on: November 26, 2013

RaMALDI: Enabling simultaneous Raman and MALDI imaging of the same tissue section.

Ethan Yang¹, Jeong Hee Kim², Caitlin M Tressler¹

¹Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.

Biosensors & Bioelectronics

|August 19, 2023

Summary

Researchers developed RaMALDI, an integrated workflow combining Raman spectroscopic imaging (RSI) and MALDI mass spectrometry imaging (MSI). This novel method enables multimodal tissue analysis on a single section, improving biomolecular discovery and diagnostics.

Keywords:

MALDI Mass spectrometry imaging Matrix Multimodal imaging Raman spectroscopy

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Last Updated: Jul 18, 2025

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

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Highly-Multiplexed Tissue Imaging with Raman Dyes

Highly-Multiplexed Tissue Imaging with Raman Dyes

Published on: April 21, 2022

Area of Science:

Biomedical Engineering
Analytical Chemistry
Molecular Pathology

Background:

Multimodal tissue imaging integrates complementary techniques for biological discovery and disease biomarker identification.
Current Raman spectroscopic imaging (RSI) and MALDI mass spectrometry imaging (MSI) require separate sample preparations, leading to image disparities.
Achieving fused images from RSI and MALDI MSI offers spatially resolved, sensitive, and specific biomolecular information.

Purpose of the Study:

To develop a streamlined, integrated multimodal imaging workflow combining RSI and MALDI MSI.
To overcome limitations of separate sample preparations in multimodal tissue imaging.
To enable simultaneous acquisition of molecular information from a single tissue section.

Main Methods:

Development of the RaMALDI workflow integrating RSI and MALDI MSI.
Application of a single sample preparation protocol for both imaging modalities.
Analysis of various tissue types using the integrated RaMALDI workflow.

Main Results:

Successful integration of molecular information from RSI and MALDI MSI on the same tissue section.
Demonstration of RaMALDI's capability to provide fused multimodal images.
Validation of the workflow across diverse tissue samples.

Conclusions:

RaMALDI offers a streamlined approach for multimodal tissue imaging.
The integrated workflow enhances the potential for discoveries in cell biology, biomedicine, and pathology.
RaMALDI is poised to advance tissue diagnostics through comprehensive molecular profiling.