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Comparative Study on Anti-Inflammatory Effect of Polysaccharides from Vinegar-Baked Radix Bupleuri Using Different
Ni Wei1, Xiaoshuang Wang1, Yayun Wu1,2
1State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Ammonia-assisted extraction (KA) yields the most anti-inflammatory polysaccharides from vinegar-baked Radix Bupleuri (VBCPs). This method optimizes yield, anti-inflammatory properties, and nanoparticle formation for VBCPs.
Area of Science:
- Pharmacology
- Natural Products Chemistry
- Biochemistry
Background:
- Radix Bupleuri polysaccharides (VBCPs) exhibit anti-inflammatory properties.
- Extraction methods significantly influence VBCP characteristics and efficacy.
- Understanding structure-activity relationships is crucial for optimizing VBCP applications.
Purpose of the Study:
- To investigate the impact of five extraction methods on VBCP physiochemical properties and anti-inflammatory effects.
- To identify the optimal extraction method for obtaining potent anti-inflammatory VBCPs.
- To elucidate structure-activity relationships governing VBCP anti-inflammatory actions.
Main Methods:
- Five extraction techniques were used: hot water (HW), ultrasound-assisted (UA), enzyme-assisted (EA), citric acid-assisted (CA), and ammonia-assisted (KA).
- Physiochemical properties (yield, sugar content, molecular weight, particle size) and anti-inflammatory effects (IL-10, NO, TNF-α inhibition) were analyzed.
- Structure-activity correlations were examined.
Main Results:
- Ammonia-assisted extraction (KA) yielded the highest VBCP yield, Ara content, and IL-10 secretion enhancement.
- VBCP-EA had the largest molecular weight and Man content, with poor NO inhibition.
- VBCP-UA showed higher Gal content, lower molecular weight, and comparable NO/TNF-α inhibition to KA and CA.
- All VBCPs formed nanoparticles, with KA VBCPs exhibiting the smallest particle size.
- Positive correlations were found between Ara content and NO inhibition/IL-10 secretion.
- Positive correlations were observed between Rha/Gal A content and TNF-α inhibition, while Glu content showed a negative correlation.
Conclusions:
- Ammonia-assisted extraction (KA) is an efficient method for producing VBCPs with significant anti-inflammatory activity.
- Extraction method critically influences VBCP yield, characteristics, and biological function.
- Molecular weight, sugar composition (Ara, Man, Rha, Gal A, Glu), and particle size are key determinants of VBCP anti-inflammatory effects.
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