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Ischemic Stroke ll: Pathophysiology

Ischemic Stroke ll: Pathophysiology

An ischemic stroke occurs when a cerebral blood vessel becomes obstructed, most often by a thrombus or embolus, interrupting the delivery of oxygen and glucose to brain tissue. Because neurons rely on continuous aerobic metabolism, energy failure begins within minutes of reduced perfusion. The region receiving the least blood flow becomes the infarct core, an area of irreversible cellular death. Surrounding this core lies the penumbra, a zone of hypoperfused but still viable tissue that is...

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Related Experiment Video

Updated: Jul 17, 2026

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Published on: April 2, 2013

Platelet morphology, ultrastructure and function changes in acute ischemic stroke patients based on structured

Bingxin Yang¹, Xifeng Wang², Xiaoyu Hu³

¹Wuhan Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China.

|August 21, 2023

Summary

Acute ischemic stroke (AIS) patients exhibit altered platelet granule morphology. Structured Illumination Microscopy (SIM) revealed changes reversed by antiplatelet drugs, with 2MeSAMP showing greater efficacy than Aspirin in vitro.

Keywords:

Acute ischemic stroke Dense granule Platelet Structured illumination microscopy α-granule

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Area of Science:

Hematology
Neurology
Biomedical Imaging

Background:

Acute ischemic stroke (AIS) is a leading global cause of mortality.
Platelet function and morphology are critical in AIS pathogenesis.
Understanding platelet ultrastructure offers insights into AIS mechanisms.

Purpose of the Study:

To assess platelet morphology, ultrastructure, and function in AIS patients using Structured Illumination Microscopy (SIM).
To investigate the effects of antiplatelet agents on platelet granule changes in AIS.
To compare the in vitro efficacy of 2MeSAMP and Aspirin in AIS platelet models.

Main Methods:

Collected platelet-rich plasma (PRP) from AIS patients and healthy controls.
Utilized SIM to visualize dense and alpha-granules in platelets under various conditions (rest, thrombin, drug treatments).
Quantified granule characteristics and measured platelet factor 4 (PF4) release via ELISA.

Main Results:

AIS patients displayed reduced platelet granule number and area compared to controls.
Platelet alpha-granules in AIS patients concentrated into fluorescent blocks after thrombin stimulation.
Antiplatelet drug treatment, particularly 2MeSAMP, reversed these granule aggregations in vitro.

Conclusions:

AIS is associated with significant alterations in platelet granule ultrastructure.
SIM provides a novel method for observing platelet changes in AIS.
Findings suggest potential for SIM-based diagnostics and therapeutic monitoring in AIS and antiplatelet drug efficacy.