A 3D-Printed Offline Nano-ESI Source for Bruker MS Instruments.

Area of Science:

• Analytical Chemistry
• Mass Spectrometry
• Biochemistry

Background:

• Nanoelectrospray ionization (nano-ESI) is vital for analyzing small analyte amounts.
• Offline nano-ESI sources are crucial for native mass spectrometry of proteins and complex mixtures.
• A readily available nano-ESI source for Bruker instruments was lacking.

Purpose of the Study:

• To develop and present a 3D-printable nano-ESI source for Bruker mass spectrometers.
• To provide an accessible solution for native mass spectrometry of proteins and complex samples on Bruker platforms.

Main Methods:

• Designed and fabricated a 3D-printable nano-ESI source.
• Adapted the source for seamless integration with Bruker mass spectrometers featuring an ionBooster ESI source.
• Performed minor, reversible modifications to existing Bruker hardware.

Main Results:

• Successfully demonstrated the utility of the 3D-printable nano-ESI source.
• Acquired high-resolution mass spectrometry spectra for small molecules, intact proteins, and complex biological samples.
• Validated performance in both negative and positive ion modes across two different Bruker instruments.

Conclusions:

• The 3D-printable nano-ESI source effectively bridges the gap for Bruker instrument users.
• This accessible technology facilitates advanced mass spectrometry analyses of diverse analytes.
• The developed source offers a versatile and customizable solution for nano-ESI applications.