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Updated: Jul 22, 2026

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
Published on: May 21, 2008
Simple chitin-based cell culture platform for production of biopharmaceuticals
Katsuhiko Kida1, Daisuke Hatanaka2, Masataka Minami2
1Biological Research Laboratories, Nissan Chemical Corporation, Saitama, Japan. kidak@nissanchem.co.jp.
Objectives:
Gene therapy using viral vectors and antibody-based therapies continue to expand within the pharmaceutical market. We evaluated whether Cellhesion® VP, a chitin-based material, can be used as 3D culture platform for cell lines used for the production of antibodies and viral vectors.
Results:
The results of Cell Counting Kit-8 assay and LDH assay revealed that Cellhesion® VP had no adverse effect to Human Embryonic Kidney (HEK) 293, A549 and Chinese hamster ovary (CHO) DG44-Interferon-β (IFN) cells. Cell growth analyses showed that Cellhesion® VP supported the 3D culture of HEK293, A549 and CHO DG44- IFN-β cells with a spherical morphology. Importantly, subculture of these cell lines on Cellhesion® VP was easily performed without trypsinization because cells readily transferred to newly added scaffold. Our data also suggest that CHO DG44-IFNβ, cultured on Cellhesion® VP secreted IFNβ stably and continuously during the culture period.
Conclusions:
Cellhesion® VP provides a simple and streamlined expansion culture system for the production of biopharmaceuticals.
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