Related Experiment Video
Updated: Jul 18, 2025

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments
Published on: June 27, 2022
Square beams for optimal tiling in TEM
Eugene Yd Chua1, Lambertus M Alink1, Mykhailo Kopylov1
1Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY 10027.
Abstract:
Imaging large fields-of-view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large field-of-view is either imperfect or results in uneven exposures, which is a problem on dose-sensitive samples. Here we introduce a square electron beam that can be easily retrofitted in existing microscopes and demonstrate its application showing it can tile nearly perfectly and deliver cryo-EM imaging with resolution comparable to conventional setups.
More Related Videos
Related Concept Videos
Transmission Electron Microscopy
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Preparation of Samples for Electron Microscopy
Overview of Electron Microscopy

