Solution and solid-state ³³ S NMR studies of ³³ S-labeled taurine

Sulfur-33(³³ S) stable-isotope labeled taurine, 2-aminoethanesulfonic acid, has been synthesized, and a series of solution and solid-state ³³ S nuclear magnetic resonance (NMR) experiments at 14.1 and 18.8 T, respectively, have been carried out at room temperature. The single peak of a solution ³³ S NMR spectrum in 0.1-mM [³³ S]-taurine in D₂ O can be observed with the signal-to-noise (S/N) ratio of 9 in 40,000 scans, which paves the way toward in vivo analysis of pharmacokinetics and metabolism of ³³ S-labeled taurine. Undistorted magic-angle-spinning (MAS) and static ³³ S NMR spectra of polycrystalline [³³ S]-taurine are observed with sufficient S/N ratios for analysis, and the magnitudes of ³³ S EFG and CS tensors can be obtained.