Related Experiment Video
Updated: Jul 18, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
High-throughput sequencing yields a complete mitochondrial genome of Emberiza godlewskii (aves, emberidae)
Jiande Li1, Lingling Da1, Xinkang Bao2
1College of Life Sciences, Northwest Normal University, Lanzhou, P.R. China.
Abstract:
Emberiza godlewskii (Taczanowski, 1874) is a passerine bird of eastern Asia which belongs to the genus Emberiza in the bunting family Emberizidae. Obtaining the complete mitochondrial genome sequence of E. godlewskii is helpful to understand the species delimitation for further study of E. cia/godlewskii complex. The circular genome (16,839 bp in length) contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 control region. The base composition shows that purine percentage (52.87%) is slightly higher than pyrimidine (47.13%). The phylogenetic analysis based on the published data of the mitochondrial genome showed that E. godlewskii is closely related to E. cioides. This new mitochondrial genome data will provide more essential molecular data for further study related to E. cia/godlewskii complex.
Related Concept Videos
Animal Mitochondrial Genetics
Export of Mitochondrial and Chloroplast Genes
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

