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Electrochemical sensor based on bio-inspired molecularly imprinted polymer for sofosbuvir detection.

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Area of Science:

  • Electrochemistry
  • Polymer Science
  • Analytical Chemistry

Background:

  • Molecularly imprinted polymers (MIPs) offer tailored recognition capabilities for various analytes.
  • Methyldopa, a functional monomer, possesses versatile chemical groups suitable for creating selective MIPs.
  • Accurate detection of sofosbuvir (SFB) is crucial for therapeutic monitoring and quality control.

Purpose of the Study:

  • To fabricate a selective MIP sensor for the detection of sofosbuvir (SFB) using methyldopa as a functional monomer.
  • To investigate the interaction between methyldopa and SFB through experimental methods.
  • To evaluate the sensor's performance, including sensitivity, selectivity, and applicability in real-world samples.

Main Methods:

  • Electropolymerization of methyldopa onto a disposable pencil graphite electrode (PGE) in the presence of SFB as a template.
  • Characterization of the fabricated sensor using X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (ESI), and cyclic voltammetry (CV).
  • Indirect detection of SFB using differential pulse voltammetry (DPV) with a ferrocyanide/ferricyanide redox probe.

Main Results:

  • The developed MIP sensor demonstrated a reproducible and linear response for SFB detection within a wide dynamic range (1.0 × 10-11 M to 1.0 × 10-13 M).
  • Achieved a low limit of detection (LOD) of 3.1 × 10-14 M for SFB.
  • Exhibited high selectivity for SFB against structurally similar compounds and common interfering drugs.

Conclusions:

  • The methyldopa-based MIP sensor provides a highly sensitive and selective platform for SFB detection.
  • The sensor's ability to function in pharmaceutical dosage forms and spiked human plasma samples highlights its practical utility.
  • This research contributes a valuable tool for the accurate quantification of SFB in various matrices.