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Updated: Jul 18, 2025

Imaging Integrin Tension and Cellular Force at Submicron Resolution with an Integrative Tension Sensor
Published on: April 25, 2019
Imaging and quantitative analysis of integrin-dependent cell-matrix adhesions
Anne-Marieke D van Stalborch1, Andrew G Clark2, Arnoud Sonnenberg3
1Amsterdam University Medical Center, 1066 CX Amsterdam, the Netherlands.
Abstract:
Integrin-dependent cell-extracellular matrix adhesion is essential for wound healing, embryonic development, immunity, and tissue organization. Here, we present a protocol for the imaging and quantitative analysis of integrin-dependent cell-matrix adhesions. We describe steps for cell culture; virus preparation; lentiviral transduction; imaging with widefield, confocal, and total internal reflection fluorescence microscopy; and using a script for their quantitative analysis. We then detail procedures for analyzing adhesion dynamics by live-cell imaging and fluorescence recovery after photobleaching (FRAP). For complete details on the use and execution of this protocol, please refer to Margadant et al. (2012),1 van der Bijl et al. (2020),2 Amado-Azevedo et al. (2021).3.
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