Differences in the long chain (sphingoid) base composition of sphingomyelin from rats bearing Morris hepatoma 7777

The long chain bases of sphingomyelin from Morris hepatoma 7777 and host and control livers were analyzed by capillary gas liquid chromatography. Sphingosine (18:1) was the major long chain base of control livers (66.5%) and hepatomas (65.6%), but hepatomas also had a high percentage (9.3 vs 4.4) of the 16:1 homolog. Host liver had the most unusual long chain base composition, with ca. equal 16:1 (24.4%) and 18:1 (21.4%) and high amounts of 20-carbon bases (9.2% 20:0 and 15.3% 20:1). These differences may be related to the aberrant fatty acid metabolism known to occur in tumor-bearing animals. Such large perturbations in the long chain base composition of hepatic sphingomyelin are unprecedented and could have a major impact on the properties of host membranes.