Glyceride synthesis by a cell-bound lipase from Aspergillus niger

A cell-bound lipase from the mould Aspergillus niger was isolated and purified to homogeneity as judged by disc electrophoresis on a Sephadex G-75 column. Molecular weight determination and electrophoretic mobility (Rf value) indicated that the enzyme differed from that secreted extracellularly. The cell-bound lipase showed a higher capacity than the extracellular enzyme for glyceride synthesis, and reacted in an opposite manner in the synthetic reaction compared with the extracellular enzyme.