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Circ_0088212 Targeting miR-576-5p/FKBP1A Axis Inhibits Osteosarcoma Progression

Xuan Yang1, Peng Sun2

  • 1Joint Sugery, Puren Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, Hubei, China.

Annals of Clinical and Laboratory Science
|August 25, 2023
PubMed
Abstract

Insights

Circular RNA circ_0088212 inhibits osteosarcoma progression by regulating the miR-576-5p/FKBP1A axis. Overexpression of circ_0088212 suppressed tumor growth, proliferation, and migration while enhancing apoptosis in osteosarcoma cells.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Circular RNAs (circRNAs) are key gene expression regulators.
  • The specific role of circRNAs in osteosarcoma (OS) requires further elucidation.

Purpose of the Study:

  • To investigate the function of circ_0088212 in osteosarcoma (OS).

Main Methods:

  • Quantification of circ_0088212, miR-576-5p, and FKBP1A via qRT-PCR.
  • Cellular function assays (proliferation, migration, apoptosis) and in vivo xenograft experiments.
  • Western blotting for apoptosis-related proteins (Bcl-2, Bax) and dual-luciferase/RNA immunoprecipitation assays to confirm molecular interactions.

Main Results:

  • circ_0088212 expression was downregulated in OS cells and tissues.
  • Overexpression of circ_0088212 inhibited OS cell proliferation and migration, and increased apoptosis.
  • circ_0088212 overexpression suppressed tumor growth in vivo, mediated by the miR-576-5p/FKBP1A axis.

Conclusions:

  • circ_0088212 acts as a tumor suppressor in osteosarcoma.
  • The miR-576-5p/FKBP1A axis is a key downstream pathway regulated by circ_0088212 in OS.
  • circ_0088212 holds potential as a therapeutic target for osteosarcoma.
Keywords:
FKBP1AOsteosarcomacirc_0088212miR-576-5p

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