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Updated: Jul 18, 2025
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
Published on: April 6, 2012
MicroRNA-1289 Functions as a Novel Tumor Suppressor in Oral Squamous Cell Carcinoma
Koh-Ichi Nakashiro1, Norihiko Tokuzen1, Masato Saika1
1Department of Oral and Maxillofacial Surgery, Ehime University Graduate School of Medicine, Toon 791-0295, Japan.
Abstract:
Recently, numerous tumor-suppressive microRNAs (TS-miRs) have been identified in human malignancies. Here, we attempted to identify novel TS-miRs in oral squamous cell carcinoma (OSCC). First, we transfected human OSCC cells individually with 968 synthetic miRs mimicking human mature miRs individually, and the growth of these cells was evaluated using the WST-8 assay. Five miR mimics significantly reduced the cell growth rate by less than 30%, and the miR-1289 mimic had the most potent growth inhibitory effect among these miRs. Subsequently, we assessed the in vivo growth-inhibitory effects of miR-1289 using a mouse model. The administration of the miR-1289 mimic-atelocollagen complex significantly reduced the size of subcutaneously xenografted human OSCC tumors. Next, we investigated the expression of miR-1289 in OSCC tissues using reverse transcription-quantitative PCR. The expression level of miR-1289 was significantly lower in OSCC tissues than in the adjacent normal oral mucosa. Furthermore, 15 genes were identified as target genes of miR-1289 via microarray and Ingenuity Pathway Analysis (IPA) microRNA target filtering. Among these genes, the knockdown of magnesium transporter 1 (MAGT1) resulted in the most remarkable cell growth inhibition in human OSCC cells. These results suggested that miR-1289 functions as a novel TS-miR in OSCC and may be a useful therapeutic tool for patients with OSCC.
Insights
Researchers identified miR-1289 as a novel tumor-suppressive microRNA (TS-miR) in oral squamous cell carcinoma (OSCC). This microRNA significantly inhibited OSCC tumor growth in vitro and in vivo, offering potential therapeutic applications.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Numerous tumor-suppressive microRNAs (TS-miRs) are recognized in human cancers.
- Oral squamous cell carcinoma (OSCC) requires novel therapeutic targets.
Purpose of the Study:
- To identify novel TS-miRs in OSCC.
- To evaluate the therapeutic potential of miR-1289 in OSCC.
Main Methods:
- Screening of 968 synthetic microRNAs (miRs) in human OSCC cells.
- In vivo evaluation of miR-1289 using a mouse xenograft model.
- Quantitative reverse transcription PCR (RT-qPCR) to assess miR-1289 expression in OSCC tissues.
- Microarray and Ingenuity Pathway Analysis (IPA) to identify miR-1289 target genes.
Main Results:
- Five miR mimics, including miR-1289, significantly inhibited OSCC cell growth.
- miR-1289 demonstrated potent in vitro and in vivo tumor growth inhibition.
- miR-1289 expression was significantly downregulated in OSCC tissues compared to normal tissues.
- Magnesium transporter 1 (MAGT1) was identified as a key target gene of miR-1289, mediating growth inhibition.
Conclusions:
- miR-1289 acts as a novel tumor-suppressive microRNA in OSCC.
- miR-1289 represents a potential therapeutic agent for OSCC treatment.
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