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Del1 Is a Growth Factor for Skeletal Progenitor Cells in the Fracture Callus
Yuxi Sun1, Tatiana Boyko2, Owen Marecic2
1Department of Surgery, University of Alabama at Birmingham, Birmingham, AL 35233, USA.
Biomolecules
|August 26, 2023
Summary
Del1 protein is crucial for bone repair by promoting the expansion of bone progenitors (BCSPs). Del1 enhances BCSP proliferation and survival, offering a potential therapeutic target for orthopedic surgeries and fracture healing.
Area of Science:
- Orthopedics
- Stem Cell Biology
- Biomaterials Science
Background:
- Bone formation and implant integration are critical in orthopedic surgery.
- Skeletal stem cells (SSCs) are key to bone repair, but downstream regulators like Bone, Cartilage, Stromal, Progenitors (BCSPs) are poorly understood.
- Del1 protein is expressed in the skeletal system but its role in bone repair was unclear.
Purpose of the Study:
- To investigate the role of Del1 in regulating BCSP expansion and bone formation following injury.
- To determine if Del1 can be a therapeutic target for enhancing bone repair and implant success.
Main Methods:
- Utilized Del1 knockout mice and wildtype (WT) littermates to assess bone formation after injury.
- Examined BCSP numbers, proliferation, and apoptosis in vivo and in vitro.
- Tested the effect of exogenous Del1 on aged human BCSPs.
Main Results:
- Del1 knockout mice showed reduced BCSP numbers, smaller callus formation, and decreased bone formation compared to WT mice.
- Del1 was found to promote BCSP proliferation and inhibit apoptosis both in vivo and in vitro.
- Exogenous Del1 treatment enhanced the proliferation of aged human BCSPs.
Conclusions:
- Del1 is a key regulator of BCSP expansion critical for bone repair after injury.
- Del1 holds therapeutic potential for improving bone formation and orthopedic implant success.
- Del1 administration may enhance fracture healing and surgical outcomes by boosting BCSP activity.
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