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Transmission-based Precautions II: Airborne and Protective Environment01:25

Transmission-based Precautions II: Airborne and Protective Environment

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Updated: Jun 25, 2026

Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts
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Isochoric Supercooling Organ Preservation System.

Gabriel Năstase1, Florin Botea2,3, George-Andrei Beșchea1

  • 1Department of Building Services, Faculty of Civil Engineering, Transilvania University of Brasov, 500152 Brasov, Romania.

Bioengineering (Basel, Switzerland)
|August 26, 2023
PubMed
Summary
This summary is machine-generated.

This study presents a novel organ preservation system using isochoric (constant volume) supercooling. The technology successfully maintained pig livers in a supercooled state for up to 48 hours without ice formation, avoiding cryoprotectants.

Keywords:
cryopreservationisochoric systemlarge organ preservationliverporcine/pig modelsubfreezing temperaturessupercooling

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Area of Science:

  • Biomedical Engineering
  • Cryobiology
  • Organ Transplantation

Background:

  • Current organ preservation methods often rely on cryoprotectants, which can be toxic.
  • Long-term preservation of large organs at subfreezing temperatures without ice formation remains a challenge.

Purpose of the Study:

  • To introduce a novel organ preservation system based on isochoric (constant volume) supercooling.
  • To evaluate the system's ability to maintain biological organs in a metastable supercooled state without ice nucleation.
  • To provide technical designs and protocols for researchers.

Main Methods:

  • Development of an organ preservation system utilizing isochoric supercooling.
  • Implementation of a control system based on isochoric freezing thermodynamics and pressure monitoring.
  • Conducting sham experiments using whole pig livers to assess supercooling stability.

Main Results:

  • Sustained supercooling without ice nucleation in pig liver tissue for 24 and 48 hours.
  • Demonstrated the potential for cryoprotectant-free preservation of large organs.
  • Provided detailed technical designs and usage protocols for the isochoric supercooling device.

Conclusions:

  • The isochoric supercooling system shows promise for long-term, cryoprotectant-free organ preservation.
  • The technology offers real-time control over the preservation process.
  • Further research is needed to confirm the transplantability of organs preserved with this method.