Related Experiment Video

Updated: Jul 18, 2025

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer
04:20

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

967

E2F8 knockdown suppresses cell proliferation and induces cell cycle arrest via Wnt/β-Catenin pathway in ovarian

Meiyin Zhang1, Ye Xu1, Yongjian Zhang1

  • 1Department of Gynecology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China.

The Chinese Journal of Physiology
|August 28, 2023
PubMed

Insights

This study identifies E2F8 as a key gene in ovarian cancer development. Silencing E2F8 inhibits tumor growth and progression by affecting the Wnt/β-catenin pathway, suggesting E2F8 as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Ovarian cancer is a leading cause of cancer death in women, with its underlying mechanisms not fully understood.
  • Identifying novel therapeutic targets is crucial for improving patient outcomes.

Purpose of the Study:

  • To investigate potential molecular targets for ovarian cancer therapy.
  • To elucidate the role of differentially expressed genes in ovarian cancer pathogenesis.

Main Methods:

  • Analysis of two Gene Expression Omnibus microarray datasets to identify differentially expressed genes (DEGs).
  • Utilized R package limma for DEG identification, followed by Gene Ontology (GO), KEGG pathway, and protein-protein interaction (PPI) network analyses.
  • Investigated the functional role of identified hub genes, specifically E2F8, through knockdown experiments and pathway analysis.

Main Results:

  • Identified 95 overlapping DEGs across two datasets, with Wnt signaling pathway and cell cycle enrichment.
  • Determined E2F8 as a top hub gene, significantly associated with poor prognosis in ovarian cancer patients.
  • E2F8 knockdown suppressed ovarian cancer cell proliferation, induced cell cycle arrest, and inhibited the Wnt/β-catenin signaling pathway.

Conclusions:

  • E2F8 plays a significant role in ovarian cancer development and progression.
  • The Wnt/β-catenin signaling pathway is implicated in E2F8's function.
  • E2F8 represents a promising therapeutic target for ovarian cancer treatment.
Keywords:
Bioinformatic analysisE2F8Wnt/β-catenin pathwaycell cycleovarian cancer

More Related Videos

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

6.8K
Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence08:55

Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence

Published on: November 2, 2014

12.3K

Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.4K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.8K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.6K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
7.5K