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Updated: Jul 17, 2025
Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
Published on: April 20, 2018
Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages
Clara Jana-Lui Busch1, Sethuraman Subramanian1, Javier Linares1
1Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Dresden, Germany.
Abstract:
Alveolar macrophages (AM) are resident macrophages of the lung and play important roles in the maintenance of tissue homeostasis as well as host defense. Here, we describe how they can be harvested from murine lungs, expanded in vitro, and transduced with lentiviral vectors.
Insights
This study details a method for isolating, culturing, and genetically modifying alveolar macrophages (AM) from mouse lungs using lentiviral vectors for research purposes.
Area of Science:
- Immunology
- Cell Biology
- Pulmonary Medicine
Background:
- Alveolar macrophages (AM) are critical immune cells residing in the lung alveoli.
- They are essential for maintaining lung tissue homeostasis and defending against pathogens.
Purpose of the Study:
- To establish a reproducible protocol for the isolation and expansion of murine alveolar macrophages.
- To demonstrate the feasibility of genetically modifying these cells using lentiviral vectors.
Main Methods:
- Harvesting alveolar macrophages from murine lungs.
- In vitro expansion of isolated alveolar macrophages.
- Transduction of alveolar macrophages with lentiviral vectors.
Main Results:
- Successful isolation and expansion of viable alveolar macrophages in culture.
- Demonstrated efficient transduction of alveolar macrophages with lentiviral vectors, enabling genetic modification.
Conclusions:
- This protocol provides a valuable tool for studying alveolar macrophage function.
- Genetically modified alveolar macrophages can be utilized for advanced research in lung immunity and disease.
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