Related Experiment Video
Updated: Jul 17, 2025
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
The necessity for improving lipid testing reagents: A real world study
Chuan Zheng1, Weiyan Zhou2, Ruyi Zhou3
1Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China; Department of Clinical Laboratory, The First People's Hospital of Shuangliu District, Chengdu/West China (Airport) Hospital, Sichuan University, Chengdu, Sichuan, China.
Background:
We investigated the interference of vitamin C (VitC), glycerol fructose, lipoprotein X (LpX) and lipemia on the analysis of serum lipids.
Methods:
Serum were collected from 44 patients with VitC infusion, serum lipid concentrations before and after VitC auto-oxidation were compared. Serum of 31 patients with glycerol fructose infusion were collected, triglycerides (TG) measured by glycerol blanking and non-blanking reagents were compared. Forty-four serum samples suspected to contain LpX were collected, LDL-C measured by reagents from five manufacturers were compared. Lipemia samples were collected, LDL-C measured using five different reagents were compared. The interference rate was considered unacceptable if it was greater than 1/2 total allowable error (TEa).
Results:
In patients with VitC infusion, the interference rates of TG and total cholesterol (TC) were -59% (-123%, -28%) and -15% (-21%, -11%), respectively. In patients with glycerol fructose infusion, the interference rate of TG was 13% (4%, 113%). LpX interference led to increased LDL-C results for most reagents. Lipemia caused great interference with LDL-C analysis.
Conclusion:
VitC, glycerol fructose, LpX and lipemia significantly interfered with lipid assays. The reagent formulation should be improved to get reliable results.
