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Updated: Jul 17, 2025
05:48
Rapid Generation of Amyloid from Native Proteins In vitro
Published on: December 5, 2013
6.2K
Phenol-soluble modulins form amyloids in contact with multiple surface chemistries
Laurent Marichal1, Lucie Bagnard1, Olivier Sire2
1Université Grenoble Alpes, CNRS, Grenoble-INP LMGP, Grenoble F-38000, France.
Biochimica Et Biophysica Acta. General Subjects
|August 28, 2023
Summary
Phenol-soluble modulins (PSMs) from Staphylococcus aureus readily adsorb to surfaces and form amyloids. This adaptability suggests a role in biofilm adhesion and structural integrity.
Area of Science:
- Microbiology
- Biomaterials Science
- Biophysics
Background:
- Functional amyloids are crucial in microbial communities.
- Staphylococcus aureus secretes phenol-soluble modulins (PSMs) that form amyloid structures within biofilms.
- The precise function of these PSM amyloids remains unclear.
Purpose of the Study:
- To investigate the bioadhesive and biofilm stabilization potential of PSM amyloids.
- To analyze PSM adsorption and amyloid formation on various abiotic surfaces.
Main Methods:
- Surface plasmon resonance imaging
- Fluorescence aggregation kinetics
- Fourier-transform infrared (FTIR) spectroscopy
- Evaluation of PSM interactions with diverse surface chemistries.
Main Results:
- PSMs demonstrate significant adsorption capabilities, even on low-binding surfaces.
- Surface chemistries do not impede the aggregation potential of PSMs into amyloids.
- PSM adsorption and amyloid formation are versatile across different tested surfaces.
Conclusions:
- PSMs exhibit high adaptability for bioadhesion due to their surface adsorption properties.
- The inherent amyloidogenic potential of PSMs is maintained irrespective of surface interactions.
- These findings suggest a potential role for PSMs in enhancing biofilm adhesion and structural integrity.
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