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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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RNA Preparation and RNA-Seq Bioinformatics for Comparative Transcriptomics.

Antonio Rodríguez-García1,2, Alberto Sola-Landa3,4, Carlos Barreiro3,5

  • 1Área de Microbiología, Departamento de Biología Molecular, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, León, Spain. arodg@unileon.es.

Methods in Molecular Biology (Clifton, N.J.)
|August 29, 2023
PubMed
Summary

This study details RNA extraction and bioinformatics protocols for RNA-sequencing (RNA-seq) to identify differentially expressed genes. It aims to make this powerful transcriptomic analysis accessible for microbiology labs.

Keywords:
BioconductorDifferential expressionRNA extractionRNA-seqTranscriptomics

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Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Genomics

Background:

  • Differential gene expression analysis is crucial in transcriptomics.
  • RNA-sequencing (RNA-seq) offers superior accuracy, dynamic range, and transcript detection compared to RT-qPCR and microarrays.
  • Many microbiology labs have not adopted RNA-seq due to perceived complexity or cost.

Purpose of the Study:

  • To provide a detailed protocol for RNA extraction.
  • To introduce bioinformatics analysis for RNA-seq data.
  • To enable the generation of differential gene expression results.

Main Methods:

  • RNA extraction protocol.
  • Bioinformatics pipeline for RNA-seq data analysis.
  • Differential gene expression analysis.

Main Results:

  • A comprehensive protocol for RNA extraction is presented.
  • An introductory guide to bioinformatics analysis for RNA-seq data is provided.
  • The protocol facilitates the identification of differentially expressed genes.

Conclusions:

  • RNA-seq is a powerful tool for transcriptomic analysis in microbiology.
  • This protocol simplifies RNA extraction and bioinformatics analysis.
  • Increased adoption of RNA-seq in microbiology labs is encouraged.