Related Experiment Video
Updated: Jul 17, 2025
Cell-Free DNA Integrity Analysis in Urine Samples
Published on: January 5, 2017
CYBA as a Potential Biomarker for Renal Cell Carcinoma: Evidence from an Integrated Genetic Analysis
Chi-Fen Chang1, Shu-Pin Huang2,3,4,5, Yu-Mei Hsueh6,7
1Department of Anatomy, School of Medicine, China Medical University, Taichung, Taiwan, R.O.C.
Background/Aim:
Oxidative stress plays an important role in various pathogenic processes, and disruption in the coordinated production of NADPH oxidase (NOX)-derived reactive oxygen species has been associated with carcinogenesis. However, little is known about whether genetic variants in NOX can contribute to the development of renal cell carcinoma (RCC).
Patients And Methods:
This study aimed to bridge this knowledge gap by analysing the association of 10 single-nucleotide polymorphisms in the phagocyte NOX genes, CYBA and CYBB, with RCC risk and tumour characteristics in 630 RCC patients and controls. Differential gene expression and patient prognosis analyses were performed using gene expression data obtained from public databases.
Results:
Multivariate analysis and multiple testing corrections revealed the A allele of rs7195830 in CYBA to be a significant risk allele for RCC, compared to the G allele [odds ratio (OR)=1.70, 95% confidence interval (CI)=1.27-2.26, p<0.001]. A pooled analysis of 17 renal cancer gene expression datasets revealed a higher CYBA expression in RCC than in normal tissues. Moreover, high CYBA expression was associated with advanced tumour characteristics and worse patient prognosis.
Conclusion:
CYBA might play an oncogenic role in RCC and serve as a predictive indicator of patient prognosis.
Insights
Genetic variants in the CYBA gene are linked to increased renal cell carcinoma (RCC) risk. Higher CYBA expression in tumors correlates with advanced disease and poorer patient prognosis, suggesting an oncogenic role.
Area of Science:
- Oncology
- Genetics
- Molecular Biology
Background:
- Oxidative stress and NADPH oxidase (NOX) dysregulation are implicated in carcinogenesis.
- The specific role of genetic variants in NOX genes in renal cell carcinoma (RCC) development remains largely unexplored.
Purpose of the Study:
- To investigate the association between single-nucleotide polymorphisms (SNPs) in phagocyte NOX genes (CYBA and CYBB) and RCC risk.
- To evaluate the correlation of CYBA gene expression with RCC tumor characteristics and patient prognosis.
Main Methods:
- Analysis of 10 SNPs in CYBA and CYBB genes in 630 RCC patients and controls.
- Utilized public gene expression datasets for differential gene expression and survival analyses.
- Performed multivariate analysis with multiple testing corrections.
Main Results:
- The 'A' allele of rs7195830 in the CYBA gene was identified as a significant risk factor for RCC (OR=1.70, p<0.001).
- Pooled analysis of 17 datasets showed elevated CYBA expression in RCC tissues compared to normal tissues.
- High CYBA expression correlated with advanced tumor stage and unfavorable patient prognosis.
Conclusions:
- The CYBA gene may possess oncogenic properties in the context of RCC.
- CYBA expression levels could serve as a potential predictive biomarker for RCC patient outcomes.
