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Published on: November 15, 2017
Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC-MS) Purification Systems
Michael Henry1, Paula Meleady2,3
1National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland.
Abstract:
Liquid chromatography/mass spectrometry (LC/MS) has become a routine powerful technology in clinical proteomic studies for protein identification, protein characterization, and the discovery of biomarkers. In this chapter, we describe two protocol methods to analyze clinical patient samples using a resin-based depletion column followed by either protein In-Gel enzymatic digestion or protein In-Solution enzymatic digestion using a simple kit-based approach (i.e., using the PreOmics iST sample preparation kit), followed by analysis using one-dimensional reverse-phase chromatography (RPC) or high pH reversed-phase peptide fractionation.
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