Mouse intraductal modeling of primary ductal carcinoma in situ
Stefan J Hutten1, Fariba Behbod2, Colinda L G J Scheele3
1Division of Molecular Pathology, The Netherlands Cancer Institute, 1066CX Amsterdam, The Netherlands; Oncode Institute, Amsterdam, The Netherlands.
Abstract:
Mouse intraductal modeling enables efficient in vivo propagation of pre-invasive breast cancer lesions and provides a suitable micro-environment for creating patient-derived tumor xenograft models of estrogen-receptor-positive breast cancer. Here, we present a protocol for mouse intraductal modeling of primary ductal carcinoma in situ (DCIS). We describe steps for processing primary DCIS tissues and performing intraductal injections. We then detail procedures for processing intraductal lesions for 3D whole-mount imaging or serial transplantation using magnetic bead sorting. For complete details on the use and execution of this protocol, please refer to Hutten et al. (2023).1.
